KMID : 1188320200140010100
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Gut and Liver 2020 Volume.14 No. 1 p.100 ~ p.107
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Mesenchymal Stem Cells Decrease Oxidative Stress in the Bowels of Interleukin-10 Knockout Mice
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Jung Kyong-Jin
Lee Gun-Woo Lee Tae-Jin Kim Joo-Young Sung Eon-Gi Kim Seong-Yong Jang Byung-Ik Song In-Hwan
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Abstract
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Background/Aims: Inflammatory bowel disease (IBD) is an autoimmune disease characterized by chronic inflammation mainly in the large intestine. The interleukin-10 knockout (IL-10 KO) mouse is a well-known animal model of IBD that develops spontaneous intestinal inflammation resembling Crohn¡¯s disease. Oxidative stress is considered to be the leading cause of cell and tissue damage. Reactive oxygen species (ROS) can cause direct cell injury and/or indirect cell injury by inducing the secretion of cytokines from damaged cells. This study evaluated the effects of mesenchymal stem cell (MSC) on the progression of IBD.
Methods: In this study, human bone marrow-derived MSCs were injected into IL-10 KO mice (MSC). Oxidative stress and inflammation levels were evaluated in the large intestine and compared with those in control IL-10 KO mice (CON) and normal wild-type control mice (Wild).
Results: The levels of ROS (superoxide and hydrogen peroxidase) and a secondary end-product of lipid peroxidation (malondialdehyde) were considerably higher in the CON, while superoxide dismutase and catalase levels were lower in the MSC. Inflammation-related marker (interferon-¥ã, tumor necrosis factor-¥á, IL-4, and CD8) expression and inflammatory histological changes were much less pronounced in MSC than in CON.
Conclusions: MSCs affect the redox balance, leading to the suppression of IBD.
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KEYWORD
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Inflammatory bowel diseases, Mesenchymal stem cells, Oxidative stress, Interleukin-10 knockout KO mouse
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